Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth

Otros

Date

2016-06-01

Registration in:

Journal of Microbiological Methods, v. 125, p. 40-42.

1872-8359

0167-7012

http://hdl.handle.net/11449/172809

10.1016/j.mimet.2016.03.018

2-s2.0-84962921676

2-s2.0-84962921676.pdf

Author

Universidade Estadual Paulista (Unesp)

Institutions

Universidade Estadual Paulista (Brasil)

Abstract

This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.

Subjects

Candida albicans

Cell survival

HEPES

Keratinocytes

Staphylococcus aureus

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