Artículos de revistas
Quantity, organization, and distribution of chloroplast microsatellites in all species of eucalyptus with available plastome sequence
Fecha
2018-01-01Registro en:
Crop Breeding and Applied Biotechnology, v. 18, n. 1, p. 97-102, 2018.
1984-7033
1518-7853
10.1590/1984-70332018v18n1a13
S1984-70332018000100097
2-s2.0-85041107145
S1984-70332018000100097.pdf
Autor
Universidade Estadual do Centro-Oeste (UNICENTRO)
Universidade Estadual Paulista (Unesp)
Institución
Resumen
In this study, we quantify and document the distribution and organization of cpSSRs in the chloroplast genome of 31 Eucalyptus species. Our sample included all previously sequenced plastomes of Eucalyptus species available from the NCBI online database. We processed the complete cpDNA sequences and identified mono-, di-, tri-, tetra-, penta-, and hexanucleotide cpSSRs, with the majority of cpSSRs classified as mononucleotide. After genome microsatellite selection, we evaluated the microsatellites for coding and non-coding regions and cpSSRs were predominantly identified in non-coding regions of cpDNA for all nucleotide types. Penta- and hexanucleotide cpSSRs were the least frequent types of microsatellites. We also developed and virtually amplified 60 primers pairs that can be used in studies of Eucalyptus species. Thus, these cpSSR regions can be used in studies assessing the ecology, breeding, and conservation of the genus.