Artículos de revistas
Synthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesis
Fecha
2016-06-01Registro en:
Protein and Peptide Letters, v. 23, n. 6, p. 544-547, 2016.
1875-5305
0929-8665
2-s2.0-84974735668
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac-Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 ôM for HaCaT and 500 ôM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx-KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.