Artículos de revistas
Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry
Fecha
2018-01-01Registro en:
Journal Of Analytical Methods In Chemistry. London: Hindawi Ltd, 10 p., 2018.
2090-8865
10.1155/2018/4869487
WOS:000424640100001
WOS000424640100001.pdf
Autor
Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (Unesp)
Universidade Federal Fluminense (UFF)
Institución
Resumen
A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol.L-1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coeffcients, above 0.995. Linear ranges of the analytes, in mg L-1, were of 0.018-18 (gallic acid), 0.146-19 (vanillic acid), 0.13-17 (caffeic acid), 0.016-16 (ferulic acid), and 0.008-17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 mu g.L-1 and precision varied in 1.73-3.78% interval. Concentrations of 19 +/- 0.51mg.L-1 and 7.8 +/- 2.5 mg.L-1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.