Artículos de revistas
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats
Fecha
2010-03-01Registro en:
Pharmaceutical Biology, v. 48, n. 3, p. 300-305, 2010.
1388-0209
10.3109/13880200903122202
WOS:000274886200011
7579144597096184
6065636367717382
4605992512582464
0421571920582994
0000-0002-8159-4853
0000-0002-4470-5171
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
This study evaluated the influence of Psidium cattleianum Sabine (Myrtaceae) and Myracrodruon urundeuva Allemão (Anacardiaceae) aqueous extracts on S. mutans counts and dental enamel micro-hardness of rats submitted to a cariogenic challenge. Sixty Wistar rats were distributed in three groups and received water (control) or aqueous extracts of Psidium cattleianum or Myracrodruon urundeuva as hydration solution. Initially the animals had their sublingual and submandibular salivary glands surgically removed and the parotid ducts ligated. Then the rats were inoculated with 106 CFU of Streptococcus mutans ATCC 35668 and were fed with a cariogenic diet. To detect and quantify the presence of S. mutans, oral biofilms were sampled and microbial DNA was extracted and submitted to amplification by means of real-time PCR (Polymerase Chain Reaction). After seven weeks the animals were sacrificed and enamel demineralization was analyzed by cross-sectional micro-hardness. Both extracts produced a significant reduction on S. mutans counts and decreased the enamel demineralization. It can be concluded that the extracts tested had a significant effect on S. mutans in oral biofilm of the rats, decreasing S. mutans accumulation and enamel demineralization.