Artículos de revistas
Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis
Fecha
2015-08-01Registro en:
Protein Expression And Purification. San Diego: Academic Press Inc Elsevier Science, v. 112, p. 15-20, 2015.
1046-5928
10.1016/j.pep.2015.04.006
WOS:000355572200003
9162508978945887
0000-0003-2460-1145
Autor
Universidade Estadual Paulista (Unesp)
Universidade Federal de Minas Gerais (UFMG)
Institución
Resumen
The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable economical loss. Cold shock proteins are conserved in many bacteria and eukaryotic cells and they help to restore normal cell functions after cold shock in which some appear to have specific functions at normal growth temperature as well.Cold shock protein A from C pseudotuberculosis was expressed in Escherichia coli and purified. The thermal unfolding/refolding process characterized by circular dichroism, differential scanning calorimetry and NMR spectroscopy techniques indicated that the refolding process was almost completely reversible. (C) 2015 Elsevier Inc. All rights reserved.