A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

Artículos de revistas

Fecha

2015-05-01

Registro en:

Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.

1517-8382

http://hdl.handle.net/11449/128702

10.1590/S1517-838246120130851

S1517-83822015000100113

WOS:000355769900013

S1517-83822015000100113.pdf

1425748916849376

0000-0001-5627-8616

Autor

Universidade Estadual Paulista (Unesp)

Institución

Universidade Estadual Paulista (Brasil)

Resumen

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

Materias

Nitrile hydratase

Amidase

Nitrilase

Amidase inhibitor

High-throughput screening

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