Artículos de revistas
Gabaergic and opioid receptors mediate the facilitation of NaCl intake induced by alpha(2)-adrenergic activation in the lateral parabrachial nucleus
Fecha
2015-02-01Registro en:
Behavioural Brain Research. Amsterdam: Elsevier Science Bv, v. 278, p. 535-541, 2015.
0166-4328
10.1016/j.bbr.2014.10.007
WOS:000347586300066
1023597870118105
339253755971890
Autor
Universidade Estadual Paulista (Unesp)
Universidade Federal de Ouro Preto (UFOP)
Institución
Resumen
Alpha(2)-adrenergic, gabaergic or opioidergic activation in the lateral parabrachial nucleus (LPBN) increases sodium intake. In the present study, we investigated the effects of single or combined blockade of opioidergic and gabaergic receptors in the LPBN on the increase of 0.3 M NaCl intake induced by alpha(2)-adrenoceptor activation in the LPBN. Male Holtzman rats (n = 5-9/group) with cannulas implanted bilaterally in the LPBN were treated with the diuretic furosemide (10 mg/kg b wt.) combined with low dose of the angiotensin converting enzyme inhibitor captopril (5 mg/kg b wt.) subcutaneously. Bilateral injections of moxonidine (alpha(2)-adrenergic/imidazoline receptor agonist, 0.5 nmol) into the LPBN increased furosemide + captopril-induced 0.3 M NaCl intake (25.8 +/- 1.4, vs. vehicle: 3.8 +/- 1.1 ml/60 min). The opioidergic receptor antagonist naloxone (100 nmol) or the GABA(A) receptor antagonist bicuculline (5 nmol) injected into the LPBN partially reduced the increase of 0.3 M NaCl intake produced by LPBN moxonidine (11.8 +/- 4.0 and 22.8 +/- 4.5, respectively, vs. vehicle+moxonidine: 31.6 +/- 4.0 ml/60 min, respectively). Similar to the treatment with each antagonist alone, the combined injections of naloxone (100 nmol) and bicuculline (5 nmol) into the LPBN also partially reduced moxonidine effects on 0.3 M NaCl intake (15.5 +/- 6.5 ml/60 min). The GABA(B) receptor antagonist saclofen (5 nmol) injected into the LPBN did not change the effects of moxonidine on 0.3 M NaCl intake (24.3 +/- 17.8 ml/120 min). These results suggest that the increase of 0.3 M NaCl intake by alpha(2)-adrenergic receptor activation in the LPBN is partially dependent on GABA(A) and opioid receptor activation in this area.