Artículos de revistas
Immunoreactivity of synthetic peptides derived from proteins of Cryptococcus gattii
Fecha
2014-07-01Registro en:
Future Microbiology. London: Future Medicine Ltd, v. 9, n. 7, p. 871-878, 2014.
1746-0913
10.2217/FMB.14.49
WOS:000341098600010
Autor
Univ Fed Piaui
Univ Esdadual Piaui
Universidade Federal de Minas Gerais (UFMG)
Fundacao Oswaldo Cruz FIOCRUZ
Univ Fed Rio Grande do Sul
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & methods: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination. Results: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity. Conclusion: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.