Artículos de revistas
Effects of autohemotherapy on hematological responses in Wistar female rats Autohemotherapy in rats
Fecha
2013Registro en:
HealthMED, v. 7, n. 4, p. 1256-1261, 2013.
1840-2291
ISSN18402291-2013-07-04-1256-1261.pdf
6796970691432850
8456421836597174
Autor
Faculdade de Medicina do ABC (FMABC)
Universidade Estadual Paulista (Unesp)
Universidade Federal de São Paulo (UNIFESP)
Institución
Resumen
Introduction: Autohemotherapy is a type of treatment that have acquired an opposite role and have presented its efficiency strived by the medical community for many reasons. In this study we aimed to evaluate the effects of authohaemotherapy on hematological response. Method: We used Wistar female rats (300g). The study consisted in a control group and a treatment group, blood samples were collected at the first day and at the eighth day after the application. In the both groups we collected 300 μl of blood from each rat through a syringe with a previously prepared solution of 30 μl of sodium citrate 3.2%. In the autohemotherapy group the blood sample was immediately injected in the quadriceps muscle on the back of the thigh hind limb. Rats from the control group did not receive intramuscular blood application. The cellular count was done through flow cytometry and the samples were dosed for immunoglobulin. Results: In the both groups we observed increased production of erytrocites, hemoglobin and platelet (p<0.05). However, there was reduction of basophil in the control group and reduction of lymphocyte, monocyte and neutrophil in the both groups. No effects were observed in IgA, IgG and IgM levels. Conclusion: Autohemotherapy did not influence hematological responses in Wistar female rats.