Artículos de revistas
HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity
Fecha
2014Registro en:
Química Nova. Sociedade Brasileira de Química, v. 37, n. 7, p. 1122-1127, 2014.
0100-4042
10.5935/0100-4042.20140193
S0100-40422014000700003
S0100-40422014000700003.pdf
3538253640602977
0000-0003-3032-2556
Autor
Universidade Estadual Paulista (Unesp)
Universidade Santa Cecília
Universidade Federal de Mato Grosso Instituto de Ciências Naturais, Humanas e Sociais
Institución
Resumen
This article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid).