Artículos de revistas
Semliki Forest Virus as a Vector: Pros and Cons for Its Use in Biopharmaceuticals Production
Fecha
2013-09-01Registro en:
Brazilian Archives of Biology and Technology. Curitiba-parana: Inst Tecnologia Parana, v. 56, n. 5, p. 859-866, 2013.
1516-8913
S1516-89132013000500018
WOS:000327507700018
S1516-89132013000500018.pdf
Autor
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Instituto Butantan
Inst Pesquisas Tecnol Estado Sao Paulo SA
Institución
Resumen
The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.