Actas de congresos
Metallomic study of mercury in fish from Amazon region - Brazil
Fecha
2013-01-01Registro en:
Proceedings Of The 16th International Conference On Heavy Metals In The Environment. Cedex A: E D P Sciences, v. 1, 2 p., 2013.
2267-1242
10.1051/e3sconf/20130126007
WOS:000326475400212
WOS000326475400212.pdf
3589137264364456
0000-0001-7264-3396
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish from Amazonas region - Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent evaluation of mercury by Thermal Decomposition and Amalgamation Coupled with Atomic Absorption (TTA-CAAS). It was found that mercury is present in 18 protein spots of dourada muscle (Brachyplatystoma rousseauxii) and 9 protein spots of pacu muscle (Myleus sp.). The protein spots in which they were determined the presence of mercury present molecular weight of 13.5 and 21.4 kDa and isoelectric point of 3.8 and 9.2, respectively.