Artículos de revistas
Osteogenesis-inducing calcium phosphate nanoparticle precursors applied to titanium surfaces
Biomedical Materials (Bristol), v. 8, n. 3, 2013.
Chalmers University of Technology
Universidade Estadual Paulista (UNESP)
This study investigated the effects of the morphology and physicochemical properties of calcium phosphate (CaP) nanoparticles on osteogenesis. Two types of CaP nanoparticles were compared, namely amorphous calcium phosphate (ACP) nano-spheres (diameter: 9-13 nm) and poorly crystalline apatite (PCA) nano-needles (30-50 nm x 2-4 nm) that closely resemble bone apatite. CaP particles were spin-coated onto titanium discs and implants; they were evaluated in cultured mouse calvarial osteoblasts, as well as after implantation in rabbit femurs. A significant dependence of CaP coatings was observed in osteoblast-related gene expression (Runx2, Col1a1 and Spp1). Specifically, the PCA group presented an up-regulation of the osteospecific genes, while the ACP group suppressed the Runx2 and Col1a1 expression when compared to blank titanium substrates. Both the ACP and PCA groups presented a more than three-fold increase of calcium deposition, as suggested by Alizarin red staining. The removal torque results implied a slight tendency in favour of the PCA group. Different forms of CaP nanostructures presented different biologic differences; the obtained information can be used to optimize surface coatings on biomaterials. © 2013 IOP Publishing Ltd.
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He, Wenxiao; Andersson, Martin; Souza, Pedro Paulo Chaves de; De Souza Costa, Carlos Alberto; Muñoz, Eduardo Mariscal; Schwartz-Filho, Humberto Osvaldo; Hayashi, Mariko; Hemdal, Amanda; Fredel, Axel; Wennerberg, Ann; Jimbo, Ryo
Escada, A. L A; Machado, J. P B; Nakazato, Roberto Zenhei; Alves Claro, A. P R
Universidade Estadual Paulista (UNESP); Laboratory of Sensors and Materials - LAS (2013-07-11)Titanium and its alloys are widely used as biomaterials due to their mechanical, chemical and biological properties. To enhance the biocompatibility of titanium alloys, various surface treatments have been proposed. In ...