Artículos de revistas
Comparison of three molecular typing methods to assess genetic diversity for Mycobacterium tuberculosis
Fecha
2013-04-01Registro en:
Journal of Microbiological Methods, v. 93, n. 1, p. 42-48, 2013.
0167-7012
1872-8359
10.1016/j.mimet.2013.01.020
WOS:000317326800008
2-s2.0-84874732670
2-s2.0-84874732670.pdf
2114570774349859
Autor
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
University of Oxford
Institución
Resumen
This study describes the comparison of three methods for genotyping of Mycobacterium tuberculosis, namely MIRU-VNTR (mycobacterial interspersed repetitive units-variable number of tandem repeats), spoligotyping and, for the first time, MLST (Multilocus Sequence Typing). In order to evaluate the discriminatory power of these methods, a total of 44 M. tuberculosis isolates obtained from sputum specimens of patients from Brazil were genotyped. Among the three methods, MLST showed the lowest discriminatory power compared to the other two techniques. MIRU-VNTR showed better discriminatory power when compared to spoligotyping, however, the combination of both methods provides the greatest level of discrimination and therefore this combination is the most useful genotyping tool to be applied to M. tuberculosis isolates. © 2013 Elsevier B.V.
Materias
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