Immunosensor based on immobilization of antigenic peptide NS5A-1 from HCV and silk fibroin in nanostructured films

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:40Z
dc.date.available2014-05-27T11:28:40Z
dc.date.created2014-05-27T11:28:40Z
dc.date.issued2013-03-19
dc.identifierLangmuir, v. 29, n. 11, p. 3829-3834, 2013.
dc.identifier0743-7463
dc.identifier1520-5827
dc.identifierhttp://hdl.handle.net/11449/74850
dc.identifier10.1021/la304404v
dc.identifierWOS:000316168400037
dc.identifier2-s2.0-84875304965
dc.description.abstractThe peptide NS5A-1 (PPLLESWKDPDYVPPWHG), derived from hepatitis C virus (HCV) NS5A protein, was immobilized into layer-by-layer (LbL) silk fibroin (SF) films. Deposition was monitored by UV-vis absorption measurements at each bilayer deposited. The interaction SF/peptide film induced secondary structure in NS5A-1 as indicated by fluorescence and circular dichroism (CD) measurements. Voltammetric sensor (SF/NS5A-1) properties were observed when the composite film was tested in the presence of anti-HCV. The peptide-silk fibroin interaction studied here showed new architectures for immunosensors based on antigenic peptides and SF as a suitable immobilization matrix. © 2013 American Chemical Society.
dc.languageeng
dc.relationLangmuir
dc.relation3.789
dc.relation1,479
dc.relation1,479
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectAntigenic peptides
dc.subjectHepatitis C virus
dc.subjectImmobilization matrices
dc.subjectLayer-by-layers
dc.subjectNanostructured Films
dc.subjectSecondary structures
dc.subjectUV-vis absorptions
dc.subjectVoltammetric sensor
dc.subjectAntigens
dc.subjectComposite films
dc.subjectImmunosensors
dc.subjectViruses
dc.subjectPeptides
dc.subjectantigen
dc.subjectfibroin
dc.subjectimmobilized protein
dc.subjectnanomaterial
dc.subjectNS 5 protein, hepatitis C virus
dc.subjectNS-5 protein, hepatitis C virus
dc.subjectpeptide fragment
dc.subjectvirus protein
dc.subjectamino acid sequence
dc.subjectanimal
dc.subjectchemistry
dc.subjectgenetic procedures
dc.subjectimmunology
dc.subjectmethodology
dc.subjectmolecular genetics
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBiosensing Techniques
dc.subjectFibroins
dc.subjectImmobilized Proteins
dc.subjectMolecular Sequence Data
dc.subjectNanostructures
dc.subjectPeptide Fragments
dc.subjectViral Nonstructural Proteins
dc.titleImmunosensor based on immobilization of antigenic peptide NS5A-1 from HCV and silk fibroin in nanostructured films
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución