Artículos de revistas
Lack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate cancer
Fecha
2013-01-16Registro en:
International Archives of Medicine, v. 6, n. 1, 2013.
1755-7682
10.1186/1755-7682-6-2
2-s2.0-84872131878
2-s2.0-84872131878.pdf
Autor
Faculdade de Medicina do ABC (FMABC)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Background: Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer. Methods. Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation. Results: There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology. Conclusion: Determination of plasma DNA by nanotechnology was not reproducible. © 2013 Moreno et al; licensee BioMed Central Ltd.