Genetic diversity of microsatellite loci in Leopardus pardalis, Leopardus wiedii and Leopardus tigrinus

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorAssociação Mata Ciliar
dc.date.accessioned2014-05-27T11:22:35Z
dc.date.available2014-05-27T11:22:35Z
dc.date.created2014-05-27T11:22:35Z
dc.date.issued2007-09-03
dc.identifierGenetics and Molecular Research, v. 6, n. 2, p. 382-389, 2007.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/69875
dc.identifierWOS:000251696400016
dc.identifier2-s2.0-34548247817
dc.identifier2-s2.0-34547569192
dc.identifier2-s2.0-34548247817.pdf
dc.identifier9991374083045897
dc.identifier3892896473273324
dc.identifier0000-0001-7083-5626
dc.description.abstractThe microsatellite loci FCA045, FCA077, FCA008, and FCA096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive Leopardus sp. The PCR-amplified products of microsatellite loci were characterized in ABI Prism 310 Genetic Analyzer. Allele numbers, heterozygosity, polymorphism information content, exclusive allele number, and shared alleles were calculated. Sixty-five alleles were found and their sizes ranged from 116 to 216 bp in four microsatellite loci. The heterozygosity ranged from 0.36 to 0.81 in Leopardus pardalis, 0.57 to 0.67 in L. tigrinus and 0.80 to 0.92 in L. wiedii. The polymorphism information content was from 0.80 to 0.88 in L. pardalis, 0.76 to 0.88 in L. tigrinus and 0.77 to 0.90 in L. wiedii. The margay (L. wiedii) showed the highest index of polymorphism among the three species in this study. These results imply that microsatellite DNA markers can help in the study of the genetic diversity of Leopardus specimens. ©FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.relation0,439
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectHeterozygosity
dc.subjectLeopardus pardalis
dc.subjectLeopardus tigrinus
dc.subjectLeopardus wiedii
dc.subjectMicrosatellite
dc.subjectmolecular marker
dc.subjectallele
dc.subjectcontrolled study
dc.subjectFelidae
dc.subjectgenetic variability
dc.subjectheterozygosity
dc.subjectmicrosatellite marker
dc.subjectmutation rate
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectLeopardus
dc.titleGenetic diversity of microsatellite loci in Leopardus pardalis, Leopardus wiedii and Leopardus tigrinus
dc.typeArtículos de revistas


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