Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus

dc.contributorDalhousie University
dc.contributorUniversidade Federal de Slo Carlos
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:19:59Z
dc.date.available2014-05-27T11:19:59Z
dc.date.created2014-05-27T11:19:59Z
dc.date.issued2000-12-01
dc.identifierHereditas, v. 133, n. 1, p. 39-46, 2000.
dc.identifier0018-0661
dc.identifierhttp://hdl.handle.net/11449/66345
dc.identifier10.1111/j.1601-5223.2000.00039.x
dc.identifier2-s2.0-0034510558
dc.identifier2-s2.0-0034510558.pdf
dc.identifier8858800699425352
dc.identifier1926863917378909
dc.identifier0000-0003-4600-0367
dc.identifier0000-0003-3534-974X
dc.description.abstractIn this study, we report the cloning and nucleotide sequence of PCR-generated 5S rDNA from the Tilapiine cichlid fish, Oreochromis niloticus. Two types of 5S rDNA were detected that differed by insertions and/or deletions and base substitutions within the non-transcribed spacer (NTS). Two 5S rDNA loci were observed by fluorescent in situ hybridization (FISH) in metaphase spreads of tilapia chromosomes. FISH using an 18S rDNA probe and silver nitrate sequential staining of 5S-FISH slides showed three 18S rDNA loci that are not syntenic to the 5S rDNA loci.
dc.languageeng
dc.relationHereditas
dc.relation0,278
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectnucleotide
dc.subjectribosome DNA
dc.subjectRNA
dc.subjectspacer DNA
dc.subjectanimal genetics
dc.subjectfemale
dc.subjectgene deletion
dc.subjectgene insertion
dc.subjectgene locus
dc.subjectgenetic analysis
dc.subjectmale
dc.subjectmetaphase chromosome
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectTilapia
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectBlotting, Southern
dc.subjectChromosome Mapping
dc.subjectChromosomes
dc.subjectDNA Primers
dc.subjectDNA, Ribosomal
dc.subjectElectrophoresis, Agar Gel
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMetaphase
dc.subjectMolecular Sequence Data
dc.subjectPerches
dc.subjectPolymerase Chain Reaction
dc.subjectRNA, Ribosomal, 5S
dc.subjectSequence Analysis, DNA
dc.subjectSequence Homology, Nucleic Acid
dc.subjectAnimalia
dc.subjectCichlidae
dc.subjectOreochromis niloticus
dc.titleNucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
dc.typeArtículos de revistas


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