Artículos de revistas
Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance
Fecha
2000-12-01Registro en:
Fresenius' Journal of Analytical Chemistry, v. 368, n. 5, p. 540-542, 2000.
0937-0633
10.1007/s002160000534
2-s2.0-0034332424
7927877224326837
0000-0003-3032-2556
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-β-D-glucopyranoside and 6-methoxykaempferol-3-O-β-D-6″(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination. © Springer-Verlag 2000.