Artículos de revistas
Electrophoretic patterns of lipopolysaccharides and antigenic analysis of soybean bradyrhizobia
Fecha
1999-01-01Registro en:
World Journal of Microbiology and Biotechnology, v. 15, n. 2, p. 185-193, 1999.
0959-3993
10.1023/A:1008859523158
WOS:000081082000006
2-s2.0-0032876599
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Several serological methods have been used for the characterization and identification of soybean bradyrhizobia. However, some problem were non-reactivity of certain strains and cross-reactivity among others. Since lipopolysaccharide (LPS) can often be used in strain identification, the objective was to investigate the antigenic properties and polyacrylamide gel electrophoretic pattern of 12 Brazilian strains of Bradyrhizobium japonicum that nodulate soybean and to compare them to standard strains. The close correlation between the LPS patterns obtained by SDS-PAGE and the serological analysis permitted us to assign the strains to nine groups different or the same as the standard strains.