Artículos de revistas
Short communication: Resazurin reducing time as an indicator of Bradyrhizobium viable cell count
Date
1998-01-01Registration in:
World Journal of Microbiology and Biotechnology, v. 14, n. 1, p. 139-141, 1998.
0959-3993
10.1023/A:1008849206064
WOS:000071535500023
2-s2.0-0031934674
Author
Universidade Estadual Paulista (Unesp)
Institutions
Abstract
A method based on the use of resazurin (RSZ) is described to determine the number of viable Bradyrhizobium japonicum cells in culture medium. The observation of RSZ reduction can be done spectrophotometrically or visually. B. japonicum strains behaved differently when the reducing time was considered. This methodology can be used to determine the number of viable cells during the liquid culture stage of inoculant production.