Artículos de revistas
Cycloactive, aneugenic and clastogenic effects of Paracoccidioides brasiliensis exoantigen in human lymphocyte cultures
Fecha
1996-12-01Registro en:
Medical Mycology, v. 34, n. 4, p. 247-250, 1996.
1369-3786
1460-2709
0268-1218
10.1080/02681219680000421
WOS:A1996VC30200003
2-s2.0-79961156179
2-s2.0-0029839930
Autor
Tringulo Mineiro Medical School (FMTM)
Universidade Estadual Paulista (Unesp)
Institución
Resumen
The in vitro effect of Paracoccidioides brasiliensis exoantigen on the human lymphocytes cell cycle and chromosomes was studied. Human peripheral blood lymphocyte cultures from ten healthy, white, non-smoking, non-related adult males (mean age 31·3 ± 8·2 years) were studied. Blood cultures were treated with three exoantigen concentrations (0·25, 2·50 and 10·00 μg ml -1). At least 1000 metaphases were analysed at each concentration, for evaluation of numerical and structural chromosome aberrations (cA) and 30 000 for mitotic index (MI). Among the treated cultures, statistically significant differences in the frequencies of MI and cA were not observed. Nevertheless, when compared with control cultures, they all showed a significantly lower frequency of MI and higher frequency of cA. It is suggested that the detected alterations were caused by the exoantigen, its fractions or its metabolites. © 1996 Informa UK Ltd All rights reserved.