Artículos de revistas
Cathodic stripping voltammetric determination of ceftazidime in urine at a hanging mercury drop electrode
Fecha
1997-09-01Registro en:
Microchemical Journal. San Diego: Academic Press Inc. Jnl-comp Subscriptions, v. 57, n. 1, p. 115-122, 1997.
0026-265X
10.1006/mchj.1997.1516
WOS:A1997YG64600012
0000-0002-2296-1393
Autor
LOUGHBOROUGH UNIV TECHNOL
Universidade Estadual Paulista (Unesp)
Institución
Resumen
A method was developed for the differential-pulse cathodic stripping voltammetric determination of ceftazidime with a hanging mercury drop electrode using its reduction peak at -0.43 V in Britton-Robinson buffer pH 4.0. The optimum accumulation potential and time were -0.15 V and up to 60 s, respectively. Linear calibration graphs were obtained from 1 x 10(-8) M and 1.5 x 10(-7) M. The limit of determination was calculated to be 5 x 10(-9) M. The coefficient of variation was 4% (n = 7) at 1 x 10(-7) M ceftazidime. The effect of various components of urine on the voltammetric response was studied, and creatinine, uric acid, urea, and glucose were shown to interfere in the method. Ceftazidime bound to human albumin gives a unique stripping peak at -0.48 V. Recoveries of 87% +/- 2% of the ceftazidime (n = 5) were obtained from urine spiked with 1.27 mu g ml(-1) using C-18 solid phase extraction cartridges. (C) 1997 Academic Press.