Artículos de revistas
Triple helix conformation of botryosphaeran, a (1 -> 3;1 -> 6)-beta-D-glucan produced by Botryosphaeria rhodina MAMB-05
Date
2008-11-21Registration in:
Carbohydrate Polymers. Oxford: Elsevier B.V., v. 74, n. 4, p. 953-956, 2008.
0144-8617
10.1016/j.carbpol.2008.04.038
WOS:000259765300032
9424175688206545
Author
Universidade Estadual Paulista (Unesp)
Univ Castilla La Mancha
Universidade Estadual de Londrina (UEL)
Institutions
Abstract
Botryosphaeran, a (1 -> 3;1 -> 6)-beta-D-glucan produced by Botryosphaeria rhodina MAMB-05, was found to be present in a triple helix conformation from helix-coil transition studies using Congo Red. The triple helix conformation was disrupted at increasing alkali concentrations. Conformational changes were also observed using phenanthrene as a fluorescent probe, and the fluorescence intensity decreased 80% in the presence of dimethyl sulfoxide. The results confirmed the triple helix conformation of botryosphaeran, an important property manifesting biological response modifying activity. (C) 2008 Elsevier Ltd. All rights reserved.