Artículos de revistas
Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
Fecha
2005-05-01Registro en:
Brazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 48, n. 3, p. 327-333, 2005.
1516-8913
10.1590/S1516-89132005000300001
S1516-89132005000300001
WOS:000229613100001
2-s2.0-27944503315
WOS000229613100001.pdf
4110421764783871
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45 degrees C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 mu mol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL)than at pH 9.0.