Artículos de revistas
Transenamel and transdentinal cytotoxicity of carbamide peroxide bleaching gels on odontoblast-like MDPC-23 cells
Fecha
2011-02-01Registro en:
International Endodontic Journal. Malden: Wiley-blackwell, v. 44, n. 2, p. 116-125, 2011.
0143-2885
10.1111/j.1365-2591.2010.01810.x
WOS:000285970800004
4517484241515548
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
P>AimTo evaluate the transenamel and transdentinal cytotoxicity of bleaching gels based on carbamide peroxide (CP) on odontoblast-like cells after different contact times of the products with enamel.MethodologyEnamel/dentine discs were obtained from bovine incisors and placed in artificial pulp chambers. Bleaching gels containing 10% or 16% CP were applied for 8 h day-1 on the enamel side of the discs during periods of 1, 7 or 14 days. Deionized water and artificial saliva served as controls. The extracts (culture medium plus bleaching gel products that diffused through the discs) were collected and applied on previously cultured MDPC-23 cells for 1 h. Cell metabolism was evaluated by the MTT assay, and the data were analysed statistically by one-way anova and Tukey's test (alpha = 0.05). Cell morphology was analysed by SEM.ResultsThere was no significant difference (P > 0.05) between the controls and the groups bleached with 10% CP gel. In the groups bleached with 16% CP gel, however, cell metabolism decreased significantly (P < 0.05) by 40.32%, 30.16% and 26.61% at 1, 7 and 14 days, respectively. There was no significant difference (P > 0.05) between 1, 7 or 14 applications of the gels for either of the CP concentrations.ConclusionRegardless of the number of applications on an enamel surface, the 10% CP bleaching gel did not cause transenamel and transdentinal cytotoxicity to the MDPC-23 cell cultures. However, diffusion of products from the 16% CP gel through enamel and dentine and cytopathic effects to the pulp cells occurred even after a single application of this product on enamel.