Artículos de revistas
Transferability of microsatellite loci from exotic Cervidae to Brazilian brocket deer (Mazama spp, Mammalia: Cervidae)
Date
2010-01-01Registration in:
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 1, p. 277-282, 2010.
1676-5680
10.4238/vol9-1gmr720
WOS:000277326200022
WOS000277326200022.pdf
Author
Universidade Estadual Paulista (Unesp)
Institutions
Abstract
Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were tested on five species of Brazilian brocket deer of the genus Mazama (M. americana, M. bororo, M. gouazoubira, M. nana, and M. nemorivaga). These primers were tested with DNA extracted from blood samples of two individuals of each species obtained from the Nucleo de Pesquisa e Conservacao de Cervideos (NUPECCE) of Universidade Estadual Paulista (UNESP). Fourteen of the 15 primers tested amplified microsatellite regions of all five species of Mazama, confirmed by sequencing of the amplified fragments. We conclude that these primers could be used for population studies of brocket deer.