Artículos de revistas
Glucocorticoids impair phagocytosis and inflammatory response against Crohn's disease-associated adherent-invasive escherichia coli
Fecha
2018Registro en:
Frontiers in Immunology, Volumen 9, Issue MAY, 2018,
16643224
10.3389/fimmu.2018.01026
Autor
Olivares Morales, Mauricio
De la Fuente, Marjorie
Dubois Camacho, Karen
Parada, Daniela
Díaz Jiménez, David
Torres-Riquelme, Alejandro
Xu, Xiaojiang
Chamorro Veloso, Nayaret
Naves, Rodrigo
González, María Julieta
Quera, Rodrigo
Figueroa,
Institución
Resumen
© 2018 Olivares-Morales, De La Fuente, Dubois-Camacho, Parada, Diaz-Jiménez, Torres-Riquelme, Xu, Chamorro-Veloso, Naves, Gonzalez, Quera, Figueroa, Cidlowski, Vidal and Hermoso. Crohn's disease (CD) is a chronic inflammatory bowel disorder characterized by deregulated inflammation triggered by environmental factors. Notably, adherent-invasive Escherichia coli (AIEC), a bacterium with the ability to survive within macrophages is believed to be one of such factors. Glucocorticoids are the first line treatment for CD and to date, it is unknown how they affect bactericidal and inflammatory properties of macrophages against AIEC. The aim of this study was to evaluate the impact of glucocorticoid treatment on AIEC infected macrophages. First, THP-1 cell-derived macrophages were infected with a CD2-a AIEC strain, in the presence or absence of the glucocorticoid dexamethasone (Dex) and mRNA microarray analysis was performed. Differentially expressed mRNAs were confirmed by TaqMan-qPCR. In add