Artículos de revistas
Mating decreases plasma levels of TGFβ1 and regulates myosalpinx expression of TGFβ1/TGFBR3 in the rat
Fecha
2014Registro en:
Molecular Reproduction and Development, Volumen 81, Issue 11, 2018, Pages 1053-1061
10982795
1040452X
10.1002/mrd.22427
Autor
Oróstica Arévalo, María Lorena
López, John
Zuñiga, Lidia M.
Utz, Daniella
Díaz, Patricia
Reuquen, Patricia
Parada Bustamante, Alexis
Cardenas, Hugo
Orihuela, Pedro A.
Institución
Resumen
© 2014 Wiley Periodicals, Inc.Mating shuts down a 2-methoxyestradiol (2ME)-dependent, non-genomic activity that is responsible for accelerating egg transport in the rat oviduct. The aims of this work were to investigate the role of TGFβ1 in this 2ME-reduced activity and to determine the effect of mating on the expression and distribution of TGFβ1 and its receptor TGFBR3 in the rat oviduct. We determined the level of TGFβ1 in the plasma and oviductal fluid at 1, 3, or 6hr during Day 1 of the oestrous cycle in unmated or mated animals. We then examined if 2ME accelerates oviductal egg transport in unmated rats that were previously treated with a neutralizing TGFβ1 antibody. The expression of Tgfb1 and Tgfbr3 mRNA and the level and distribution of TGFBR3 protein in the oviduct were also determined at these time points. Mating decreased TGFβ1 in the plasma, but not in the oviductal fluid, whereas antibody neutralization of circulating TGFβ1 did not prevent the effect of 2ME on egg transpor