Artículos de revistas
Thy-1-mediated cell-cell contact induces astrocyte migration through the engagement of αVβ3 integrin and syndecan-4
Fecha
2013Registro en:
Biochimica et Biophysica Acta - Molecular Cell Research, Volumen 1833, Issue 6, 2018, Pages 1409-1420
01674889
18792596
10.1016/j.bbamcr.2013.02.013
Autor
Kong, Milene
Muñoz, Nicolás
Valdivia, Alejandra
Alvarez, Alvaro
Herrera Molina, Rodrigo
Cárdenas, Areli
Schneider, Pascal
Burridge, Keith
Quest, Andrew F. G.
Leyton Campos, Lisette
Institución
Resumen
Cell adhesion to the extracellular matrix proteins occurs through interactions with integrins that bind to Arg-Gly-Asp (RGD) tripeptides, and syndecan-4, which recognizes the heparin-binding domain of other proteins. Both receptors trigger signaling pathways, including those that activate RhoGTPases such as RhoA and Rac1. This sequence of events modulates cell adhesion to the ECM and cell migration. Using a neuron-astrocyte model, we have reported that the neuronal protein Thy-1 engages αVβ3 integrin and syndecan-4 to induce RhoA activation and strong astrocyte adhesion to their underlying substrate. Thus, because cell-cell interactions and strong cell attachment to the matrix are considered antagonistic to cell migration, we hypothesized that Thy-1 stimulation of astrocytes should preclude cell migration. Here, we studied the effect of Thy-1 expressing neurons on astrocyte polarization and migration using a wound-healing assay and immunofluorescence analysis. Signaling molecules invol