Artículo de revista
A synthetic peptide homologous to IL-10 functional domain induces monocyte differentiation to TGF-β+ tolerogenic dendritic cells
Fecha
2011Registro en:
Immunobiology, Volumen 216, Issue 10, 2018, Pages 1117-1126
01712985
18783279
10.1016/j.imbio.2011.04.006
Autor
López, Mercedes N.
Pesce Reyes, Bárbara
Kurte, Mónica
Pérez Núñez, Claudio
Segal, Gabriela
Roa, Johanna
Aguillón Gutiérrez, Juan Carlos
Mendoza Naranjo, Ariadna
Gesser, Borbala
Larsen, Christian
Villablanca, Andrea
Choudhury, Aniruddha
Kiessling, Rolf
Salazar Onfray, Flavio
Institución
Resumen
We have previously demonstrated that IT9302, a nonameric peptide homologous to the C-terminal domain of human IL-10, mimics several effects of the cytokine including down-regulation of the antigen presentation machinery and increased sensitivity of tumor cells to NK-mediated lysis. In the present report, we have explored a potential therapeutic utility for IT9302 related to the ex vivo production of tolerogenic dendritic cells (DCs). Our results indicate that IT9302 impedes human monocyte response to differentiation factors and reduces antigen presentation and co-stimulatory capacity by DCs. Additionally, peptide-treated DCs show impaired capacity to stimulate T-cell proliferation and IFN-γ production. IT9302 exerts its effect through mechanisms, in part, distinct from IL-10, involving STAT3 inactivation and NF-κB intracellular pathway. IT9302-treated DCs display increased expression of membrane-associated TGF-β, linked to a more effective induction of foxp3+ regulatory T cells. These