Artículo de revista
P450Arom induction in isolated control endometrial cells by peritoneal fluid from women with endometriosis
Fecha
2010Registro en:
Fertility and Sterility, Volumen 94, Issue 7, 2010, Pages 2521-2527
00150282
10.1016/j.fertnstert.2010.03.036
Autor
Castro, Jazmín
Torres, Marisa
Sovino, Hugo
Fuentes, Ariel
Boric, María Angélica
Johnson, M. Cecilia
Institución
Resumen
Objective: To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on
P450Arom expression in endometrial cells.
Design: Experimental study.
Setting: University research unit.
Patient(s): Forty women of reproductive age with (n ¼ 22) or without (control; n ¼ 18) endometriosis.
Intervention(s): Peritoneal fluid and eutopic endometrial samples were obtained during surgery from women with
(n ¼ 13 and 9, respectively) and without (n ¼ 4 and 14, respectively) endometriosis.
Main Outcome Measure(s): Expression study for P450Arom, steroid factor 1 (SF-1), chicken ovalbumin upstream
transcription factor I (COUP-TFI), and COUP-TFII messenger RNA (reverse transcriptase–polymerase chain
reacion) and/or protein (immunoblot) in isolated endometrial epithelial cells transfected or not with expression
vector containing SF-1, COUP-TFI, or COUP-TFII complementary DNAs.
Result(s): Basal messenger RNA and/or protein expression of P450Arom and SF-1 were augmented in endometriosis,
and that of COUP-TF was diminished. In control cells, (Bu)2cAMP and PF-E increased P450Arom and SF-1
expression (but not COUP-TF expression) in a dose-dependent way, an effect not observed with PF-C, adsorbed
PF-E, or 10 5 M indomethacin. Transfected cells confirmed these results. Any treatments modified the studied
molecules in endometriosis cells.
Conclusion(s): These data indicate that molecules contained in PF-E favor an estrogenic microenvironment,
suggesting a role in the etiopathogenesis of endometriosis enabling the survival, maintenance, and growth of
endometrial implants in the ectopic locations.