Artículos de revistas
Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ
Fecha
2010Registro en:
Development, Volumen 137, Issue 20, 2018, Pages 3459-3468
14779129
09501991
10.1242/dev.049981
Autor
Oteiza, Pablo
Köppen, Mathias
Krieg, Michael
Pulgar, Eduardo
Farias, Cecilia
Melo, Cristina
Preibisch, Stephan
Müller, Daniel
Tada, Masazumi
Hartel, Steffen
Heisenberg, Carl-Philipp
Concha, Miguel L.
Institución
Resumen
Organ formation requires the precise assembly of progenitor cells into a functional multicellular structure. Mechanical forces probably participate in this process but how they influence organ morphogenesis is still unclear. Here, we show that Wnt11- and Prickle1a-mediated planar cell polarity (PCP) signalling coordinates the formation of the zebrafish ciliated laterality organ (Kupffer's vesicle) by regulating adhesion properties between organ progenitor cells (the dorsal forerunner cells, DFCs). Combined inhibition of Wnt11 and Prickle1a reduces DFC cell-cell adhesion and impairs their compaction and arrangement during vesicle lumen formation. This leads to the formation of a mis-shapen vesicle with small fragmented lumina and shortened cilia, resulting in severely impaired organ function and, as a consequence, randomised laterality of both molecular and visceral asymmetries. Our results reveal a novel role for PCP-dependent cell adhesion in coordinating the supracellular organisatio