Artículo de revista
Differentiation of Trypanosoma cruzi I subgroups through characterization of cytochrome b gene sequences
Fecha
2008Registro en:
Infection, Genetics and Evolution, Volumen 8, Issue 6, 2018, Pages 898-900
15671348
10.1016/j.meegid.2008.08.006
Autor
Spotorno Oyarzún, Ángel
Córdova, Luis
Solari Illescas, Aldo
Institución
Resumen
To identify and characterize chilean samples of Trypanosoma cruzi and their association with hosts, the first 516 bp of the mitochondrial cytochrome b gene were sequenced from eight biological samples, and phylogenetically compared with other known 20 American sequences. The molecular characterization of these 28 sequences in a maximum likelihood phylogram (-ln L = 1255.12, tree length = 180, consistency index = 0.79) allowed the robust identification (bootstrap % >99) of three previously known discrete typing units (DTU): DTU IIb, IIa, and I. An apparently undescribed new sequence found in four new chilean samples was detected and designated as DTU Ib; they were separated by 24.7 differences, but robustly related (bootstrap % = 97 in 500 replicates) to those of DTU I by sharing 12 substitutions, among which four were nonsynonymous ones. Such new DTU Ib was also robust (bootstrap % = 100), and characterized by 10 unambiguous substitutions, with a single nonsynonymous G to T change at s