Artículo de revista
Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-β signaling
Fecha
2007Registro en:
Journal of Cellular Physiology, Volumen 210, Issue 2, 2018, Pages 456-468
00219541
10.1002/jcp.20878
Autor
Santibanez, Juan F.
Letamendia, Ainhoa
Perez-Barriocanal, Fernando
Silvestri, Cristoforo
Saura, Marta
Vary, Calvin P.H.
Lopez-Novoa, Jose M.
Attisano, Liliana
Bernabeu, Carmelo
Institución
Resumen
The endothelial nitric oxide synthase (eNOS) is a critical regulator of cardiovascular homeostasis, whose dysregulation leads to different vascular pathologies. Endoglin is a component of the transforming growth factor beta (TGF-β) receptor complex present in endothelial cells that is involved in angiogenesis, cardiovascular development, and vascular homeostasis. Haploinsufficient expression of endoglin has been shown to downregulate endothelium-derived nitric oxide in endoglin+/- (Eng+/-) mice and cultured endothelial cells. Here, we find that TGF-β1 leads to an increased vasodilatation in Eng+/+ mice that is severely impaired in Eng+/- mice, suggesting the involvement of endoglin in the TGF-β regulated vascular homeostasis. The endoglin-dependent induction of eNOS occurs at the transcriptional level and is mediated by the type I TGF-β receptor ALK5 and its downstream substrate Smad2. In addition, Smad2-specific signaling is upregulated in endoglin-induced endothelial cells, whereas i