Artículos de revistas
Properties of voltage-gated Ca2+ currents measured from mouse pancreatic β-cells in situ
Fecha
2006Registro en:
Biological Research, Volumen 39, Issue 3, 2018, Pages 505-520
07176287
07169760
10.4067/S0716-97602006000300012
Autor
Mears, David
Rojas, Eduardo
Institución
Resumen
We used the single-microelectrode voltage-clamp technique to record ionic currents from pancreatic β-cells within intact mouse islets of Langerhans at 37C, the typical preparation for studies of glucose-induced "bursting" electrical activity. Cells were impaled with intracellular microelectrodes, and voltage pulses were applied in the presence of tetraethylammonium. Under these conditions, a voltage-dependent Ca2+ current (ICav), containing L-type and non-L-type components, was observed. The current measured in situ was larger than that measured in single cells with whole-cell patch clamping, particularly at membrane potentials corresponding to the action potentials of β-cell electrical activity. The temperature dependence of ICav was not sufficient to account for the difference in size of the currents recorded with the two methods. During prolonged pulses, the voltage-dependent Ca2+ current measured in situ displayed both rapid and slow components of inactivation. The rapid component