Artículos de revistas
Alterations of rat liver mitochondrial oxidative phosphorylation and calcium uptake by benzo[a]pyrene
Fecha
2004Registro en:
Toxicology and Applied Pharmacology, Volumen 198, Issue 1, 2018, Pages 1-10
0041008X
10.1016/j.taap.2004.02.013
Autor
Salazar, Inés
Pavani, Mario
Aranda, Waldo
Maya Arango, Juan
Morello Casté, Antonio
Ferreira, Jorge
Institución
Resumen
We report that oxidative phosphorylation and Ca2+ uptake processes are enhanced in liver mitochondria isolated from benzo[a]pyrene (B[a]P)-treated rats. The carcinogen did not affect either the respiratory control index or the Ca2+ control ratio. B[a]P treatment increased the oxidation rate of several substrates that donate electrons at the level of all three coupling sites, either the ADP- or Ca2+-stimulated rates or those observed after ADP or Ca2+ exhaustion. However, the efficiency of energy coupling was maintained because both ADP/O and Ca2+/site ratios remained unchanged. The electron flow through NADH-oxidase, NADH-duroquinone reductase, NADH-juglone reductase, NADH-cytochrome c reductase, succinate-cytochrome c reductase, and cytochrome c oxidase was enhanced by B[a]P; however, succinate dehydrogenase activity was not affected. All these effects depended on the time post B[a]P administration, with a greater increase close to 48 h after administration of the carcinogen. The cont