Artículos de revistas
Vitamin E but not 17β-estradiol protects against vascular toxicity induced by β-amyloid wild type and the dutch amyloid variant
Fecha
2002Registro en:
Journal of Neuroscience, Volumen 22, Issue 8, 2018, Pages 3081-3089
02706474
Autor
Muñoz, Francisco J.
Opazo, Carlos
Gil-Gómez, Gabriel
Tapia, Gladys
Fernández, Virginia
Valverde, Miguel A.
Inestrosa, Nibaldo C.
Institución
Resumen
Amyloid β-peptide (Aβ) fibril deposition on cerebral vessels produces cerebral amyloid angiopathy that appears in the majority of Alzheimer's disease patients. An early onset of a cerebral amyloid angiopathy variant called hereditary cerebral hemorrhage with amyloidosis of the Dutch type is caused by a point mutation in Aβ yielding AβGlu22→Gln. The present study addresses the effect of amyloid fibrils from both wild-type and mutated Aβ on vascular cells, as well as the putative protective role of antioxidants on amyloid angiopathy. For this purpose, we studied the cytotoxicity induced by Aβ1-40 Glu22→Gln and Aβ1-40 wild-type fibrils on human venule endothelial cells and rat aorta smooth muscle cells. We observed that AβGlu22→Gln fibrils are more toxic for vascular cells than the wild-type fibrils. We also evaluated the cytotoxicity of Aβ fibrils bound with acetyl-cholinesterase (AChE), a common component of amyloid deposits. Aβ1-40 wild-type-AChE fibrillar complexes, similar to neurona