Artículo de revista
Dermatan sulfate proteoglycans from the mineralized matrix of the avian eggshell
Fecha
1997Registro en:
Connective Tissue Research, Volumen 36, Issue 3, 2018, Pages 175-193
03008207
10.3109/03008209709160219
Autor
Carrino, David A.
Rodriguez, Juan Pablo
Caplan, Arnold I.
Institución
Resumen
The eggshell of the chicken is a useful model to study matrix components which affect biomineralization. As an extension of our previous immunohistochemical work which suggested the presence of dermatan sulfate proteoglycans in the mineralized region of the eggshell, a study was undertaken to characterize these molecules biochemically. After demineralization with HCl and extraction with 4 M guanidinium chloride containing protease inhibitors, the extract was partitioned by anion exchange chromatography. Step elution with 0.25 M and 1.0 M sodium chloride resulted in the generation of two fractions, both of which contain chondroitinase-sensitive proteoglycans with molecular weights estimated at 200,000 by gel electrophoresis. The proteoglycans in each fraction have core proteins with molecular weights of approximately 120,000 and glycosaminoglycans with average molecular weights of 22,000. Based on differential sensitivity to chondroitinase ABC and AC II, these glycosaminoglycans contain