Artículos de revistas
Antigenemia and real time polymerase chain reaction for diagnosis of cytomegalovirus disease in HIV infected adults Antigenemia y reacción de polimerasa en cadena en tiempo real en el diagnóstico de enfermedad por citomegalovirus en adultos con virus de i
Fecha
2015Registro en:
Revista Chilena de Infectologia, Volumen 32, Issue 6, 2018, Pages 664-671
07161018
10.4067/S0716-10182015000700008
Autor
Luchsinger, Vivian
Vásquez, Patricia
Silva, Matías
José Bruno, M.
Siches, Izkia
Villarroel, Javier
Luisa Garmendia, M.
Larrañaga, Carmen
Institución
Resumen
© 2015, Sociedad Chilena de Infectologia. All rights reserved.Background: Cytomegalovirus (CMV) infection is frequent in HIV adults. It is unknown usefulness of quantitative methods for diagnosing the CMV disease in Chilean patients. Aim: To determine the performance of antigenemia and real time polymerase chain reaction (rtPCR) in the diagnosis of CMV disease in Chilean HIV adults. Method: Detection of CMV by viral isolation (AVR), antigenemia and quantitative rtPCR in HIV adults. Results: The 102 adults with suspected CMV disease had lower LTCD4 count and higher HIV viral load than 77 patients without suspicion (p < 0.05). Antigenemia and PCR were positive in 47 (46.1%) and 37 (36.3%) adults with clinical suspicion and in 2 (2.6%) and 4 (5.2%) of 77 without suspicion. The sensitivity, specificity, positive and negative predictive value of antigenemia and RPCtr were 92%, 80%, 72% and 95% and 72%, 95%, 92% and 80%, respectively. The cutoff values were ≥ 1cell (+) and ≥ 5.5 log10 copies