Artículos de revistas
Sperm phospholipid methyltransferase activity during preparation for exocytosis
Fecha
1994Registro en:
Cell Biochemistry and Function, Volumen 12, Issue 4, 2018, Pages 289-296
10990844
02636484
10.1002/cbf.290120410
Autor
Llanos Silva, Miguel
Ronco Macchiavello, Ana María
Institución
Resumen
The present report describes experiments to evaluate phospholipid methyltransferase activity in golden hamster spermatozoa incubated under different conditions. Washed cauda epididymal sperm were incubated with taurine, in the presence or absence of epinephrine. At various times, the sperm were separated, and phospholipid methyltransferase activity measured. Also, at each time, aliquots of the sperm suspension were assayed for motility, and acrosome reactions. Some sperm incubated in the presence of taurine and epinephrine were capacitated by 3·5 h, because about 40 per cent of them can undergo the acrosome reaction 10 min after addition of the fusogen lysophosphatidylcholine. In epinephrine‐free incubations the fusogen failed to stimulated acrosome reactions. On the other hand, epinephrine stimulated by twofold phospholipid methyltransferase activity from ‘0 time’ incubated sperm, in comparison to that observed in taurine‐treated cells. Enzyme activities from both taurine or epinephri