Artículos de revistas
Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe
Fecha
1992Registro en:
Epidemiology and Infection, Volumen 108, Issue 1, 2018, Pages 175-184
14694409
09502688
10.1017/S0950268800049621
Autor
Fernández, J.
Sandino, A.
Yudelevich, A.
Avendaño, L. F.
Venegas, A.
Hinrichsen, V.
Spencer, E.
Institución
Resumen
A synthetic oligodeoxynucletide of 40 nucleotides corresponding to nucleotides 33–72 of the gene coding for the viral protein VP7 of rotavirus, was used as a nucleic acid probe to develop a non-radiactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive. © 1992, Cambridge University Press. All rights reserved.