Artículo de revista
Certain large forms of circulating immunoreactive human growth hormone are in fact immunoglobulins
Fecha
1990Registro en:
Journal of Clinical Endocrinology and Metabolism, Volumen 70, Issue 3, 2018, Pages 601-605
19457197
0021972X
10.1210/jcem-70-3-601
Autor
Campino, Carmen
Szecowka, Jaroslaw
Michelsen, Harold
Serón Ferré, María
Institución
Resumen
To explain frequent discordances between serum GH levels and clinical manifestation of acromegaly, we investigated the possibility that certain immunoglobulins G (IgGs) might be responsible for the displacement of [125I]human (h) GH in the hGH RIA. We incubated dilute sera from seven active acromegalics (basal immunoreactive hGH, 22–313 μg/L) with rat adipocyte plasma membranes adsorbed on polystyrene plates. IgGs that bound to GH receptor sites in the absence and presence of 250 nM hGH (for nonspecific binding) were detected using anti-hlgG (Fc-specific) antibody conjugated with alkaline phosphatase. In this system two of the seven sera studied tested positive for IgGs against GH-binding sites (serum 4 in 1:400 dilution, and serum 7 in 1:10 dilution). We studied further the serum with the highest titer. On Sephadex G-100, most of the GH-like immunoreactivity (assayed by RIA) present in serum 4 coeluted with IgGs (assayed by immunodiffussion) as a high mol wt (≥ 150 kDa) component. To