Artículos de revistas
Role of sulfhydryl groups an the stimulatory effect of captopril on vascular prostacyclin synthesis
Fecha
1991Registro en:
European Journal of Pharmacology, Volumen 198, Issue 1, 2018, Pages 1-6
00142999
10.1016/0014-2999(91)90554-4
Autor
Guivernau, Mauricio
Armijo, Felipe
Rosas, Ramon
Institución
Resumen
The effect of captopril on vascular prostacyclin production was studied, evaluating which of its components - sulfhydryl (SH) group or proline - is responsible for this effect. Rat aortas were incubaicd with captopril (10-100 μM), 2-mercaptoethanol or proline (10 μM), and captopril plus the SH-binding reagents N-ethylmaleimide or ethacrynic acid (50 gmM). Prostacyclin was measured by radioimmunoassay of 6-keto-prostaglandin F1α Captopril stimulated prostacyclin production. This effect was associated with an enhanced conversion of arachidonate to prostacyclin and was not related to bradykinin. Since 2-mereaptoethanol increased vascular prostacyclin per se and proline did not, the stimulatory effect of captopril appears to be dependent upon the SH group; in addition, both SH Mockers, N-ethylmaleimide and ethacrynic acid, antagonized this effect. This study shows that captopril stimulates vascular prostacyclin synthesis directly and that the SH group plays a key role in this action. This