In vitro methylation of the elongation factor EF-Tu from Escherichia coli

dc.creatorToledo, Héctor
dc.creatorJerez, Carlos A.
dc.date.accessioned2019-01-29T12:39:47Z
dc.date.available2019-01-29T12:39:47Z
dc.date.created2019-01-29T12:39:47Z
dc.date.issued1985
dc.identifierFEBS Letters, Volumen 193, Issue 1, 1985, Pages 17-21
dc.identifier00145793
dc.identifier10.1016/0014-5793(85)80070-3
dc.identifierhttp://repositorio.uchile.cl/handle/2250/159640
dc.description.abstractThe in vitro methylation of the elongation factor EF-Tu from Escherichia coli was investigated. The methylation of newly synthesized EF-Tu was obtained using lambda rifd 18 DNA as template and S-adenosyl [methyl-3H]methionine as methyl donor. About 3 mol methyl residues were incorporated for every 10 mol EF-Tu synthesized. Analysis of the nature of the methyl-containing residues by protein hydrolysis followed by paper chromatography showed that both mono- and dimethyllysine were present. The methylation of EF-Tu was also studied separately from its synthesis by using cell-free systems with artificially undermethylated components.
dc.languageen
dc.sourceFEBS Letters
dc.subjectDNA-dependent system
dc.subjectElongation factor EF-Tu
dc.subjectEthionine
dc.subjectProtein methylation
dc.titleIn vitro methylation of the elongation factor EF-Tu from Escherichia coli
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución