Artículo de revista
Caries-free subjects have high levels of urease and arginine deiminase activity
Fecha
2014Registro en:
Journal of Applied Oral Science, Volumen 22, Issue 3, 2014, Pages 235-240.
16787765
16787757
10.1590/1678-775720130591
Autor
Reyes, Evelyn
Martin, Javier
Gustavo, Moncada
Neira, Miguel
Palma, Patricia
Gordan, Valeria
Oyarzo, Juan
Yevenes, Ismael
Institución
Resumen
Objectives: This study investigated the relationship between urease and arginine
deiminase system (ADS) activities and dental caries through a cross-sectional study.
Material and Methods: Urease and ADS activities were measured in saliva and plaque
samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained
from the ammonia produced by incubation of plaque and saliva samples in urea. ADS
activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate
buffer. Specific activity was defined as micromoles of ammonia per minute per milligram
of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data,
and Mann-Whitney test was used to determine the significance of the data. Results: The
specific urease activity in saliva and plaque was significantly higher in individuals with low
DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs
1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Cariesfree subjects had a higher ammonia generation activity by urease and arginine deiminase
system for both saliva and plaque samples than low caries-active subjects. High levels of
alkali production in oral environment were related to caries-free subjects.