Artículos de revistas
DNA from oral bacteria by sodium hydroxide-paper method suitable for polymerase chain reaction
Fecha
2013Registro en:
Analytical Biochemistry, Volumen 433, Issue 2, 2013, Pages 129-131.
00032697
10960309
10.1016/j.ab.2012.10.024
Autor
Lefimil, Claudia
Lozano, Carla
Morales Bozo, Irene
Plaza, Anita
Maturana, Cristian
Urzúa Orellana, Blanca
Institución
Resumen
In the oral cavity, we can find a complex mixture of microorganisms, commensals, and pathogens. The studies of normal oral microbiota, as well as the studies of much oral pathology (e.g., caries, periodontitis), involve the isolation and cultivation of these microorganisms and their molecular analysis. The aim of this study was to validate a quick, easy, efficient, and inexpensive DNA extraction method for the recovery of genomic DNA from gram-positive and gram-negative oral bacteria to be used in polymerase chain reaction amplification. This method worked great with all samples analyzed, providing an approach to extract DNA for different microorganisms.