Artículos de revistas
Conformational and physicochemical properties of quinoa proteins affected by different conditions of high-intensity ultrasound treatments
Fecha
2018Registro en:
Ultrasonics Sonochemistry, Volumen 51,
18732828
13504177
10.1016/j.ultsonch.2018.10.026
Autor
Vera, A.
Valenzuela, M. A.
Yazdani-Pedram Zobeiri, Mehrdad
Tapia, C.
Abugoch James, Lilian
Institución
Resumen
© 2018 Elsevier B.V. Quinoa proteins (QP) have promise as a potential source of novel food ingredients, and it is of great interest to know how high-intensity ultrasound (HIUS) treatments affect the properties of QP. This work aimed to study the impact of on-off time-pulses of HIUS treatments on the structural and physicochemical properties of QP; samples were treated at 5, 10, 20, and 30 min with on-off pulses of 10 s/10 s, 5 s/1 s, and 1 s/5 s). Structural changes were evaluated using PAGE-SDS, circular dichroism, fluorescence spectroscopy, and differential scanning calorimetry. Meanwhile, physicochemical properties were also examined, including solubility, Z-average, polydispersity index PDI, and Z-potential. PAGE-SDS showed the appearance of polypeptides over 190 kDa in HIUS samples-treated. All samples presented 15.6% α-helices, 31.3% β-sheets, 21.8% β-rotations, and 31.4% random coils independent of the HIUS treatment. β-Turn structures and “random coils” were not affected by HIU