Artículo de revista
Functionalization of stable fluorescent nanodiamonds towards reliable detection of biomarkers for Alzheimer's disease
Fecha
2018Registro en:
Journal of Nanobiotechnology, Volumen 16, Issue 1, 2018,
14773155
10.1186/s12951-018-0385-7
Autor
Morales-Zavala, Francisco
Casanova-Morales, Nathalie
Gonzalez, Raúl B.
Chandía-Cristi, América
Estrada, Lisbell D.
Alvizú, Ignacio
Waselowski, Victor
Guzman, Fanny
Guerrero, Simón
Oyarzún-Olave, Marisol
Rebolledo, Cristian
Rodriguez, Enrique
Armijo, Julie
Institución
Resumen
© 2018 The Author(s). Background: Stable and non-toxic fluorescent markers are gaining attention in molecular diagnostics as powerful tools for enabling long and reliable biological studies. Such markers should not only have a long half-life under several assay conditions showing no photo bleaching or blinking but also, they must allow for their conjugation or functionalization as a crucial step for numerous applications such as cellular tracking, biomarker detection and drug delivery. Results: We report the functionalization of stable fluorescent markers based on nanodiamonds (NDs) with a bifunctional peptide. This peptide is made of a cell penetrating peptide and a six amino acids long β-sheet breaker peptide that is able to recognize amyloid β (Aβ) aggregates, a biomarker for the Alzheimer disease. Our results indicate that functionalized NDs (fNDs) are not cytotoxic and can be internalized by the cells. The fNDs allow ultrasensitive detection (at picomolar concentrations of NDs) of